Our Equine Piroplasmosis Bundle combines cELISA and qPCR tests for comprehensive detection of antibodies to Babesia caballi and Theileria equi. This bundle is crucial for the official trading, import, and export of horses.
Product Overview
The bundle includes:
- Two cELISA Tests: Detect antibodies to Babesia caballi and Theileria equi. Used for official trading and movement of horses. Follows ISO/IEC 17025 standards, ensuring high quality and reliability.
- qPCR Test: Detects the genome of Babesia caballi and Theileria equi, providing highly sensitive detection of piroplasms, ideal for early infection detection and during febrile peaks.
Sample Requirements
- 5 mL of blood or serum - collect blood in a dry tube for cELISA
- 5 mL of blood - collect blood in K3 EDTA tube for qPCR
Turnaround Time
Standard Processing: Results in 2 working days after sample arrival at the laboratory. Clients are responsible for organizing and covering the costs of sending the samples to the laboratory.
What is Piroplasmosis?
Equine piroplasmosis (EP) is a tick-borne disease of horses caused by the intraerythrocytic protozoan parasites Babesia caballi and Theileria equi. These agents are transmitted through a tick vector. Infected animals may remain carriers of these blood parasites for long periods and act as sources of infection for other ticks. The parasites are found in southern Europe, Asia, countries of the Commonwealth of Independent States, Africa, Cuba, South and Central America, and parts of the southern United States.
Clinical Signs
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Incubation period: 12 to 19 days for T. equi and 10 to 30 days for B. caballi.
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Per-acute form: Rare, with only clinical observation being moribund or dead animals.
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Acute form: Fever, reduced appetite, elevated respiratory and pulse rates, congestion of mucous membranes, dark red urine, smaller and drier faecal balls, anaemia, and/or icterus.
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Subacute form: Similar to acute form but with weight loss and intermittent fever. Mucous membranes may vary from pale pink to bright yellow.
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Chronic form: Mild inappetence, poor performance, weight loss. Documented case fatality rates vary from 10–50%.
Transmission
Babesia caballi sporozoites invade red blood cells (RBCs), transforming into trophozoites which divide into merozoites, capable of infecting new RBCs. B. caballi can be found in various organs of tick vectors and transmit transovarially.
Theileria equi sporozoites, inoculated into horses via a tick bite, invade lymphocytes, develop into schizonts, and release merozoites that invade RBCs. T. equi develop in the salivary glands of the tick vector and are not transmitted transovarially. Transmission is also possible through mechanical vectors contaminated by infected blood.
Prevention
Sanitary Prophylaxis: Testing and controlling tick exposure, using repellents, acaricides, and regular inspections, controlling and eradicating the tick vector, and quarantining EP-positive animals.
Medical Prophylaxis: No current biological products are available. Antiprotozoal agents only temporarily clear T. equi from carriers.
How It Works
How It Works
- 🛒 Purchase the Test: Select and buy the test online.
- 📧 Receive Instructions: After payment confirmation, receive instructions for sample collection.
- ✨ Sample Collection: Your veterinarian collects the sample.
- 📄 Download Submission Form: Download the printable submission form here.
- 📮 Send Samples: Send to our lab by regular mail or express delivery to:
Equigerminal Lab
IPN Incubadora, Rua Pedro Nunes, EdifC
3030-199 Coimbra, PORTUGAL
- 📄 Receive Results: Get the result certificate by email. If you need assistance, contact us at support@equigerminal.pt.
More Info
View More Info
For more detailed information on the cELISA and qPCR Tests, including sample collection and submission instructions, please visit our website or contact our support team.
Visit our detailed diagnosis page for more information.
FAQs
View FAQs
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How do the cELISA and qPCR tests work? The cELISA test detects antibodies to Babesia caballi and Theileria equi, providing a highly sensitive and specific method for identifying the presence of the pathogens. The qPCR test detects the genome of these pathogens, making it ideal for early infection detection and during febrile peaks.
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What types of samples are required for the tests? The cELISA test requires 5 mL of blood or serum collected in a dry tube. The qPCR test requires 5 mL of blood collected in a K3 EDTA tube.
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How long does it take to get the test results? The turnaround time for the cELISA and qPCR tests is typically 2 working days from the receipt of the sample in the laboratory.
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What should be done if a horse tests positive for Piroplasmosis? Horses that test positive should be isolated to prevent the spread of the disease. Follow biosecurity measures and consult with a veterinarian for appropriate treatment and management.
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How can Piroplasmosis be prevented? Prevention involves testing and controlling tick exposure, using repellents, acaricides, and regular inspections, controlling and eradicating the tick vector, and quarantining EP-positive animals.
