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Salmonellosis qPCR

    Pathogen test  The PCR test detects the genome (DNA) of the Salmonella serovar abortus-equi, the bacteria responsible for Salmonellosis and abortion in equines. Sample 1 genital swabs...

    422,00 kr

      Sample RequirementsSample Requirements
      • 1 genital swabs - sterile swab 
      • 20 gr - placental or foetal tissues - sterile flask
      • 5 mL - blood - K3 EDTA tube

      Send your sample by regular mail or express delivery to:

      Equigerminal Lab HIESE
      Rua da Quinta do Sobreiro Nº25
      3230-343 Penela, Portugal

      Turnaround TimeTurnaround Time

      2 to 5 working days

      About the testAbout the test

      This qPCR test detects the genome (DNA) of Salmonella enterica serovar Abortus-equi, the bacterial pathogen responsible for salmonellosis and abortion in horses. Molecular detection provides rapid and sensitive identification of the organism in clinical samples.

      Why test?Why test?

      Testing is recommended to:

      • Investigate equine abortions caused by Salmonella Abortus-equi.
      • Support the diagnosis of equine salmonellosis.
      • Identify infected animals during disease outbreaks.
      • Assist veterinarians in implementing appropriate treatment and biosecurity measures.
      • Reduce the spread of this contagious and zoonotic pathogen.
      How it worksHow it works

      🛒 Purchase the Test: Select and buy the test online.

      📧 Receive Instructions: After payment confirmation, receive instructions for sample collection.

      Sample Collection: Your veterinarian collects the appropriate sample(s).

      📄 Download Submission Form: Download the printable submission form.

      📮 Send Samples: Send your samples by regular mail or express delivery to:

      Equigerminal Lab HIESE
      Rua da Quinta do Sobreiro Nº25
      3230-343 Penela, Portugal

      📄 Receive Results: Get your laboratory report by email.

      Clinical signsClinical signs

      Clinical signs may include:

      • Abortion in pregnant mares
      • Fever
      • Diarrhoea
      • Colic
      • Loss of appetite
      • Lethargy
      • Septicaemia
      • Laminitis following enterocolitis

      Foals may develop severe systemic disease including pneumonia, septic arthritis, meningitis and haemorrhagic diarrhoea.

      TransmissionTransmission
      • Fecal-oral spread
      • Ingestion of contaminated material (pasture, roughage, feed or water)
      • Fomites are a significant means of indirect transmission of infection
      • Intermittent shedding by subclinically infected horses
      • Aerosol transmission has been suspected in other species; evidence of this route in horses is lacking
      PreventionPrevention

      Measures Biosecurity Guidelines

      • Quarantine horses that develop diarrhoea and/or fever. If a separate stall or paddock is not available, establish barrier precautions at their current location
      • Isolate horses following significant colic episodes, impactions (notably small colon), or colic surgery to reduce environmental contamination and potential exposure of other horses should Salmonella subsequently be recovered on fecal culture
      • Prevent horses that have come in contact with known infected or clinical cases from mixing with the general population
      • Contaminated stall and equipment should have all organic material removed. Dispose of organic matter in a manner which prevents contamination of the facility (do not spread on pastures). Disinfection can be performed after all organic matter has been removed and the surfaces cleaned. Pressure washers or hoses should not be used as they can aerosolise Salmonella, potentially contaminating other parts of the facility or infecting a susceptible horse or human
      • No commercially available validated vaccine is currently marketed. For animals with positive cultures while clinically ill:
      • Before removing restrictions, following resolution of clinical signs, conduct a series of fecal cultures (see Diagnostic Sampling, Testing and Handling) to determine if all negative
      • Where culture is not performed, isolation up to 30 days may be required to minimize risk of exposure of other horses from convalescent shedding of previously infected horses following the cessation of clinical signs (fever, diarrhoea). • Isolate horse for 30 days from resident horses
      • Obtain 5 consecutive negative fecal cultures prior to releasing horse into the general population
      • Prior to entry into the general population the horse should be housed in an environment that can be thoroughly cleaned and disinfected
      • If the horse is turned out in a paddock, manure should be promptly removed and appropriately disposed of in a manner that avoids potential contamination of other areas of the facility. Caretakers should wear personal protective equipment. After the horse is released, the paddock should be harrowed to encourage drying and kept unused for 30 days
      ResultsResults

      The qPCR test reports one of the following:

      • Detected: Salmonella Abortus-equi DNA was identified in the submitted sample.
      • Not Detected: No Salmonella Abortus-equi DNA was detected.

      Results should always be interpreted together with clinical findings and veterinary examination.

      Additional informationAdditional information

      Salmonella Abortus-equi is an important cause of equine abortion and systemic disease. Because infected horses may shed the bacteria intermittently without obvious clinical signs, laboratory testing is essential for confirming infection and controlling outbreaks. Early diagnosis allows rapid implementation of biosecurity measures to protect both horses and people.

      FAQsFAQs

      Which horses should be tested?

      Pregnant mares with abortion, horses showing diarrhoea, fever or colic, and animals involved in suspected salmonellosis outbreaks should be tested.

      Which samples are accepted?

      A genital swab, placental or foetal tissue, or 5 mL of blood collected in a K3 EDTA tube.

      Can healthy horses carry Salmonella?

      Yes. Clinically healthy horses may intermittently shed Salmonella bacteria without showing clinical signs, particularly during periods of stress or illness.

      When will I receive my results?

      Results are available within 2 to 5 working days after sample arrival at the laboratory.

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