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JEV Japanese Encephalitis Virus, RT-qPCR

Name: JEV Japanese Encephalitis Virus, RT-qPCR
Brand: Equigerminal
Price: 61.5 EUR

Test that looks for the Japanese encephalitis virus, spread by mosquitoes, which attacks the brain. Pathogen test The RT-qPCR test detects the genome (RNA) of Japanese...

€61.50 Incl. VAT

About

The RT-qPCR test detects the genome (RNA) of Japanese Encephalitis Virus (JEV).

What is Japanese Encephalitis Virus?

Japanese Encephalitis occurs throughout much of Asia, including India, China and Japan.

In temperate regions, infections usually occur during late summer and autumn, when mosquito activity is highest. The virus circulates naturally between water birds and mosquitoes before spreading to pigs and occasionally to humans and horses.

Although horses can develop severe disease, they are considered dead-end hosts because the amount of virus in their blood is insufficient to infect mosquitoes.

Why test?

The RT-qPCR test is recommended for:

Detection of active Japanese Encephalitis Virus infection.
Horses showing neurological signs compatible with Japanese Encephalitis.
Investigation of suspected cases during outbreaks or in endemic areas.

Clinical signs

These include:

vary from a passing fever through to violent neurological signs and death
mild cases: off feed, sluggish and reddened or jaundiced (yellow) mucous membranes (gums)
more serious cases: lethargic with a fluctuating fever, difficulty swallowing, jaundice (yellow), pinpoint haemorrhages in mucous membranes (gums)
nervous signs such as lack of coordination, staggering, falling, aimless wandering and unpredictable behaviour may occur in serious cases
severe cases: blindness with profuse sweating and muscle trembling before collapsing and dying.

Transmission

Japanese Encephalitis Virus is transmitted by mosquitoes.

The virus naturally circulates between water birds (particularly herons and egrets) and mosquitoes. Pigs also play an important role in the transmission cycle.

Humans and horses can develop severe disease but do not transmit the virus because they do not develop sufficient levels of virus in the bloodstream to infect mosquitoes.

The incubation period is 8–10 days.

Prevention

Sanitary prophylaxis

Housing animals in-doors in screened stabling can provide protection from mosquitoes o Especially during active JE outbreaks and during peak vector activity (usually dawn to dusk) o Insecticides, repellents and fans also provide protection.
Vector control reduces transmission.
Vaccine is available for horses .
Vaccine protects horses from clinical disease and possible sequelae.

Results

The RT-qPCR test detects the genome (RNA) of Japanese Encephalitis Virus (JEV).

Results are reported as Positive or Negative for the presence of viral RNA.

Sample

5 mL - blood - K3 EDTA tube
5mL - liquor (CSF) - sterile tube

Turnaround

2 to 5 working days

How it works

🛒 Buy the test: select and buy the test online.

📧 Receive the instructions: after payment is confirmed, you receive the sample collection instructions by e-mail.

📄 Complete the form: print and complete the submission form with the animal’s identification.

✨ Collect the sample: pull 20 to 40 hair roots with the bulb and stick them in the marked area of the form, or ask your veterinarian to collect blood in a K3-EDTA tube.

📮 Send it to the laboratory: Equigerminal, S.A., HIESE, Rua da Quinta do Sobreiro, 25, Quinta Vale do Espinhal, 3230-343 Penela, PORTUGAL.

📊 Receive the report: the certified report is issued as soon as the analysis is validated.

FAQs

🛒 Purchase the Test: Select and buy the test online.

📧 Receive Instructions: After payment confirmation, receive instructions for sample collection.

✨ Sample Collection: Your veterinarian collects blood or cerebrospinal fluid (CSF), depending on the clinical case.

📄 Download Submission Form: Download the printable submission form.

📮 Send Samples: Send your samples by regular mail or express delivery to:

Equigerminal Lab HIESE
Rua da Quinta do Sobreiro Nº25
3230-343 Penela, Portugal

📄 Receive Results: Get the result certificate by email.

More

Horses are considered dead-end hosts, meaning they can become infected and develop disease but do not contribute to further transmission because viral levels in the blood are too low to infect mosquitoes.