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Dilution champagne

Name: Dilution champagne
Brand: Equigerminal
Price: 43.05 EUR

Le test ADN vérifie la présence de la mutation champagne. Champagne est un modificateur de dilution du pelage. Échantillon 30 à...

€43.05 Incl. T.V.A.

À propos du test

Le test ADN vérifie la présence de la mutation Champagne.

Champagne est un modificateur de dilution de pelage.

Pourquoi tester ?

Equigerminal propose un test pour la mutation dominante du gène champagne.

Le test ADN peut être utile dans les cas où un cheval a déjà été testé négatif pour les dilutions crème ou argent, mais semble avoir un pelage éclairci.

Le test est également utilisé pour déterminer l'homozygotie du gène champagne.

Signes cliniques

Champagne lightens red and black pigment alike, so it changes every base colour. One copy and two copies look the same. Champagne horses range from gold to dark tan and tend to have amber eyes. With no copy, the base colour shows as it is.

A black base becomes classic champagne
A chestnut base becomes gold champagne
A bay base becomes amber champagne

What fools the eye:

Gold champagne read as palomino, and amber champagne read as buckskin
Pearl, which also lightens the coat and the eye
A horse carrying both Champagne and Cream, in which the two lightenings add together
Grey, which slowly strips any of these colours away

Health. No disease is linked to Champagne.

Transmission

Champagne is dominant. One copy is enough to lighten the coat. The gene is not on a sex chromosome, so colts and fillies are the same.

What each mating can produce:

N/N x N/N gives 100% N/N
N/N x N/Ch gives 50% N/N, 50% N/Ch
N/N x Ch/Ch gives 100% N/Ch
N/Ch x N/Ch gives 25% N/N, 50% N/Ch, 25% Ch/Ch
N/Ch x Ch/Ch gives 50% N/Ch, 50% Ch/Ch
Ch/Ch x Ch/Ch gives 100% Ch/Ch

Prévention

The result lets you plan for champagne foals.

To guarantee a champagne foal. Use a parent with two copies.

To keep champagne out. Use two partners that tested negative. Because one copy always shows, a horse that is not lightened by Champagne does not carry it.

Résultats

Le test ADN vérifie la présence de la mutation champagne et présente les résultats comme l'un des suivants :

N/ – Cheval non-champagne.
N/Ch – Positif pour le gène champagne dominant, possédant une copie héritée. Le pelage sera dilué en conséquence. Transmettra le gène champagne à environ 50 % de la descendance.
Ch/ – Positif pour le gène champagne dominant, possédant deux copies héritées. Le pelage sera dilué en conséquence.

Exigences d'échantillon

30 à 40 - racines de cheveux - enveloppe ou 5 mL - sang - tube K3 EDTA

Envoyez votre échantillon par courrier ordinaire ou livraison express à :

Laboratoire Equigerminal HIESE
Rua da Quinta do Sobreiro Nº25
3230-343 Penela, Portugal

Délai d'exécution

2 à 5 jours ouvrables

Comment ça marche

🛒 Purchase the test: select and buy the test online.

📧 Receive instructions: after payment confirmation you receive sample collection instructions by e-mail.

✨ Collect the sample yourself: pull 20 to 40 hair roots with the bulb attached, or ask your veterinarian to collect blood in a K3-EDTA tube.

📄 Complete the form: print and complete the submission form with the animal identification.

📮 Send it to the laboratory: Equigerminal, S.A., HIESE, Rua da Quinta do Sobreiro, 25, Quinta Vale do Espinhal, 3230-343 Penela, PORTUGAL.

📄 Receive your report: your certified report is issued as soon as the analysis is validated.

Questions fréquentes

What does this test not detect?
It does not test Cream, Pearl, Silver, Dun, Grey, Mushroom, the base colour or any white pattern. It says nothing about health, performance or temperament.

At what age can a horse be tested?
Any age. The genotype is fixed at conception and never changes.

For Professionals

Genetic and clinical detail for veterinarians, geneticists and laboratories.

Gene / locus: SLC36A1 (solute carrier family 36 member 1), Champagne (CH) locus, ECA14.
Variant: c.188C>G, missense in exon 2. HGVS (EquCab3.0): NC_009157.3:g.26012449G>C; XM_023617382.1:c.188C>G; XP_023473150.1:p.(Thr63Arg).
Alternative designations: genomic G>C and cDNA C>G are the same change on opposite strands. Laboratory allele symbol: Ch.
Variant identifiers: rs5334475190 (EVA); OMIA variant 152.
Variant classification: not currently evaluated (ISAG/AVCG, per OMIA).
Gene function and discovery: SLC36A1 encodes an amino acid transporter. Cook et al. (2008) mapped the trait in the families of three stallions to a region of ECA14, screened genes expressed in skin and found a single change. Champagne was the first trait linked to SLC36A1 in any species.
Inheritance: autosomal dominant (OMIA code D); N/Ch and Ch/Ch are phenotypically indistinguishable.
Penetrance / expressivity: complete concordance in the discovery study: present in all 85 champagne horses and absent in all 97 non-champagne horses (Cook et al., 2008).
Breeds with documented carriers or cases: documented in American Cream Draft, American Miniature Horse, American Paint Horse, American Saddlebred, Appaloosa, Missouri Fox Trotting Horse, Quarter Horse, Tennessee Walking Horse and Kentucky Mountain Saddle Horse; first identified in Tennessee Walking Horse families. Detected in eight of 28 breeds surveyed: Missouri Fox Trotter, Rocky Mountain Horse, Miniature Horse, Gypsy Cob, Quarter Horse, Tennessee Walking Horse, Paint Horse, Shetland Pony (Avila et al., 2022), the Rocky Mountain Horse being a first report. Not detected in that study in Andalusian, Appaloosa, Arabian, Connemara Pony, Dutch Warmblood, Gypsy Vanner, Hanoverian, Icelandic Horse, Knabstrupper, Lusitano, Morgan Horse, Mustang, Norwegian Fjord Horse, Oldenburg, Percheron, Pony of the Americas, Pura Raza Espanola, Standardbred, Thoroughbred or Welsh Pony; absence in a sample does not prove absence in the breed.
Allele / carrier frequencies: allele frequency (Avila et al., 2022; 11,281 horses tested, 6,878 after removing close relatives; per-breed n not given in our sources): Missouri Fox Trotter 5.4%, Rocky Mountain Horse 2.6%, Miniature Horse 1.7%, Gypsy Cob 0.8%, Quarter Horse 0.8%, Tennessee Walking Horse 0.8%, Paint Horse 0.7%, Shetland Pony 0.2%. The Tennessee Walking Horse, the discovery breed, is now among the lowest. The authors note ascertainment bias, as samples were submitted for colour testing, so figures for less-sampled breeds may be overestimated.

Key references:

Cook D, Brooks S, Bellone R, Bailey E (2008). Missense mutation in exon 2 of SLC36A1 responsible for champagne dilution in horses. PLoS Genet 4:e1000195. doi:10.1371/journal.pgen.1000195. PMID 18802473.
Sponenberg DP, Bowling AT (1996). Champagne, a dominant color dilution of horses. Genet Sel Evol 28:457-462.
Avila F, Hughes SS, Magdesian KG, Penedo MCT, Bellone RR (2022). Breed distribution and allele frequencies of base coat color, dilution, and white patterning variants across 28 horse breeds. Genes 13:1641.
Melvin K, Ivey J, Johnston L. Equine Genetics: Basic Coat Color Inheritance. University of Tennessee Institute of Agriculture, UT Extension publication W891.