Produktformulär

CEMO Contagious Equine Metritis Organism, qPCR

    Test that looks for Taylorella equigenitalis, the germ that causes contagious equine metritis, in genital swabs. Pathogen test  This PCR test detects the genome (DNA) of...

    €73.80 Inkl. moms

      AboutAbout

      This qPCR test detects the genome (DNA) of Taylorella equigenitalis, the most common bacterium responsible for Contagious Equine Metritis (CEM), an inflammatory disease of the mare's reproductive tract that usually results in temporary infertility.

      Why test?Why test?

      Contagious equine metritis is an inflammatory disease of the proximal and distal reproductive tract of the mare caused byTaylorella equigenitalis, which usually results in temporary infertility. It is a nonsystemic infection, the effects of which are restricted to the reproductive tract of the mare.

      Clinical signsClinical signs
      • When present, general clinical signs include endometritis, cervicitis and vaginitis of variable severity and a slight to copious mucopurulent vaginal discharge.

      In mares there are two states of infection:

      • The active statein which the main outward sign is a vulval discharge, which may range from very mild to extremely profuse.
      • The carrier statein which there are no outward signs of infection. However, the mare remains capable of transmitting infection because the bacteria are established on the surface of the clitoris, the clitoral fossa and sinuses and, in the case of pneumoniae andP. aeruginosa, sometimes in the urethra and bladder.

      In stallions: (‘stallion’ means mating stallions, teasers and stallions used for AI)

      • Infected stallions do not usually show clinical signs of infection but the bacteria are present on their penis, sheath and. These stallions can infect mares during mating, teasing or AI.
      • Occasionally, the bacteria may invade the stallion’s sex glands, causing pus and bacteria to contaminate the semen.
      TransmissionTransmission
      • Direct venereal contact during natural mating presents the highest risk for the transmission ofequigenitalisfrom a contaminated stallion or an infected mare.
      • Direct venereal transmission can also take place by artificial insemination using infective raw, chilled and possibly frozen semen.
      • Indirectly, infection may be acquired through fomite transmission, manual contamination, inadequate observance of appropriate biosecurity measures at the time of breeding and at semen- collection centres. Stallions can become asymptomatic carriers ofequigenitalis.
      • The principal sites of colonisation by the bacterium are the urogenital membranes (urethral fossa, urethral sinus, terminal urethra and penile sheath).

      The sites of persistence ofequigenitalisin the majority of carrier mares are the clitoral sinuses and fossa and infrequently the uterus.

      • Foals born of carrier mares may also become carriers.
      • The organism can infect equid species other than horses, e.g. donkeys.
      PreventionPrevention

      If infection withequigenitalisis suspected in any mare, stallion or teaser on the basis of clinical signs, all breeding activities must cease immediately.

      The affected horse(s) should be isolated and swabbed by the attending veterinary surgeon.

      Arrange swabbing of any at risk horse.

      Disinfect all equipment used for breeding procedures.

      Inform all owners of mares booked to the stallion, including any which have already left the premises;

      Inform people to whom semen from the stallion has been sent;

      Arrange for one straw from every ejaculate of stored semen from infected and at risk stallions to be tested by a laboratory. If a straw from any ejaculate is infected, all straws from that ejaculate should be destroyed;

      Any at risk pregnant mare must be foaled in isolation. The placenta must be incinerated. Foals born to these mares should be swabbed three times, at intervals of not less than seven days, before three months of age.

      Any mares with an abnormal vaginal exudate, or returning to oestrus prematurely, should be investigated and managed as though infected withequigenitalisuntil results of laboratory testing prove otherwise.

      If carriers ofequigenitalisare detected, the organism can be eliminated by treatment with systemic and/or local antibiotics combined with antiseptic washing of the sites of persistence in the mare and the stallion.

      SampleSample
      • 3 genital swabs - swab Amies transport with charcoal.
      • Clitoral fossa– use standard swab with Amies culture and transport system
      • Clitoral sinuses swabbed – use Minitip Amies culture and transport system. Openings to the sinuses are on the dorsum of the clitoris - the central one is usually always present whereas the lateral sinuses may be multiple or not be present. Swab all that are present.
      • Either cervical (closed cervix if pregnant or mid-cycle) or endometrial(while in estrus or true anestrus) swab – use guarded 25” swab.
      TurnaroundTurnaround

      2 to 5  working days

      How it worksHow it works

      🛒 Buy the test: select and buy the test online.

      📧 Receive the instructions: after payment is confirmed, you receive the sample collection instructions by e-mail.

      📄 Complete the form: print and complete the submission form with the animal’s identification.

      ✨ Collect the sample: pull 20 to 40 hair roots with the bulb and stick them in the marked area of the form, or ask your veterinarian to collect blood in a K3-EDTA tube.

      📮 Send it to the laboratory: Equigerminal, S.A., HIESE, Rua da Quinta do Sobreiro, 25, Quinta Vale do Espinhal, 3230-343 Penela, PORTUGAL.

      📊 Receive the report: the certified report is issued as soon as the analysis is validated.

      FAQsFAQs

      Who collects the sample? Your veterinarian. See Sample for the material and the volume this test requires.

      How should the sample be sent? Keep it cool and send it by tracked courier as soon as it is collected. Shipping is arranged and paid for by the client.

      What happens if the sample arrives unsuitable? We contact you before processing and ask for a new sample.

      Can other tests be added to the same sample? Often yes, if the material and the volume allow it. Ask us before shipping.

      How long does it take? See Turnaround. The count starts when the sample arrives at the laboratory, not when you order.

      MoreMore

      This PCR testdetectsthe genome (DNA) of Taylorella equigenitalis the most comum bacteria responsible for theContagious Equine Metritis

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