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Japanese Encephalitis Virus RTqPCR

    Pathogen test  The RT-qPCR test detects the genome (RNA) of Japanese Encephalitis virus (JEV). Sample 5 mL - blood - K3 EDTA tube 5mL - liquor (CSF) -...

    £54.00

      Sample RequirementsSample Requirements
      • 5 mL - blood - K3 EDTA tube
      • 5mL - liquor (CSF) - sterile tube

      Send your sample by regular mail or express delivery to:

      Equigerminal Lab HIESE
      Rua da Quinta do Sobreiro Nº25
      3230-343 Penela, Portugal

      Turnaround TimeTurnaround Time

      2 to 5 working days

      About the testAbout the test

      The RT-qPCR test detects the genome (RNA) of Japanese Encephalitis Virus (JEV).

      What is Japanese Encephalitis Virus?

      Japanese Encephalitis occurs throughout much of Asia, including India, China and Japan.

      In temperate regions, infections usually occur during late summer and autumn, when mosquito activity is highest. The virus circulates naturally between water birds and mosquitoes before spreading to pigs and occasionally to humans and horses.

      Although horses can develop severe disease, they are considered dead-end hosts because the amount of virus in their blood is insufficient to infect mosquitoes.

      Why test?Why test?

      The RT-qPCR test is recommended for:

      • Detection of active Japanese Encephalitis Virus infection.
      • Horses showing neurological signs compatible with Japanese Encephalitis.
      • Investigation of suspected cases during outbreaks or in endemic areas.
      How it worksHow it works

      What does the RT-qPCR test detect?

      The RT-qPCR test detects the genome (RNA) of Japanese Encephalitis Virus (JEV).

      Which samples can be submitted?

      The test can be performed using 5 mL of blood collected in a K3 EDTA tube or 5 mL of cerebrospinal fluid (CSF) collected in a sterile tube.

      Can infected horses transmit the virus?

      No. Horses are considered dead-end hosts because they do not develop enough virus in their bloodstream to infect mosquitoes.

      How is Japanese Encephalitis transmitted?

      The virus is transmitted by infected mosquitoes and naturally circulates between mosquitoes and water birds. Pigs also play an important role in the transmission cycle.

      How long does it take to receive the results?

      Results are available within 2 to 5 working days after sample arrival at the laboratory.

      Clinical signsClinical signs

      These include:

      • vary from a passing fever through to violent neurological signs and death
      • mild cases: off feed, sluggish and reddened or jaundiced (yellow) mucous membranes (gums)
      • more serious cases: lethargic with a fluctuating fever, difficulty swallowing, jaundice (yellow), pinpoint haemorrhages in mucous membranes (gums)
      • nervous signs such as lack of coordination, staggering, falling, aimless wandering and unpredictable behaviour may occur in serious cases
      • severe cases: blindness with profuse sweating and muscle trembling before collapsing and dying.
      TransmissionTransmission

      Japanese Encephalitis Virus is transmitted by mosquitoes.

      The virus naturally circulates between water birds (particularly herons and egrets) and mosquitoes. Pigs also play an important role in the transmission cycle.

      Humans and horses can develop severe disease but do not transmit the virus because they do not develop sufficient levels of virus in the bloodstream to infect mosquitoes.

      The incubation period is 8–10 days.

      PreventionPrevention

      Sanitary prophylaxis

      • Housing animals in-doors in screened stabling can provide protection from mosquitoes o Especially during active JE outbreaks and during peak vector activity (usually dawn to dusk) o Insecticides, repellents and fans also provide protection.
      • Vector control reduces transmission.
      • Vaccine is available for horses .
      •  Vaccine protects horses from clinical disease and possible sequelae.
      ResultsResults

      The RT-qPCR test detects the genome (RNA) of Japanese Encephalitis Virus (JEV).

      Results are reported as Positive or Negative for the presence of viral RNA.

      Additional informationAdditional information

      Horses are considered dead-end hosts, meaning they can become infected and develop disease but do not contribute to further transmission because viral levels in the blood are too low to infect mosquitoes.

      FAQsFAQs

      🛒 Purchase the Test: Select and buy the test online.

      📧 Receive Instructions: After payment confirmation, receive instructions for sample collection.

      ✨ Sample Collection: Your veterinarian collects blood or cerebrospinal fluid (CSF), depending on the clinical case.

      📄 Download Submission Form: Download the printable submission form.

      📮 Send Samples: Send your samples by regular mail or express delivery to:

      Equigerminal Lab HIESE
      Rua da Quinta do Sobreiro Nº25
      3230-343 Penela, Portugal

      📄 Receive Results: Get the result certificate by email.

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