Name: Diluzione crema
Brand: Equigerminal
Price: 43.05 EUR

Informazioni sul test

Il gene della diluizione crema ha effetti diversi su differenti colori di base.

Per ottenere il ‘nome tipo’ esatto della diluizione crema del cavallo, si consiglia di eseguire questo test insieme ai geni Extension e Agouti.

Perché testare?

Il test è utile quando è necessaria una conferma genetica o per definire i cavalli con diluizione crema rispetto ad altri geni con effetti simili (come la diluizione champagne e il grigio).

Eseguire questo test confermerà se un cavallo è diluito crema. Come detto, il gene della diluizione crema ha effetti variabili su diversi colori di base. Per ottenere il ‘nome tipo’ esatto della diluizione crema del cavallo (ad es. Buckskin, Palomino, Cremello…) si consiglia di eseguire questo test insieme al fattore rosso e all’agouti.

Segni clinici

Cream lightens the coat. How it looks depends on the base colour and on the number of copies. With no copy, the base colour shows as it is.

One copy: red pigment lightened.

Chestnut becomes palomino: a golden body with a white or flaxen mane and tail
Bay becomes buckskin: a golden body with the black points kept
Black becomes smoky black, which usually looks like an ordinary black

Two copies: red and black pigment lightened. The coat goes pale cream, the skin is pink and the eyes are blue.

Chestnut becomes cremello
Bay becomes perlino: a cream body with slightly darker, rust-tinged points
Black becomes smoky cream

What fools the eye:

Smoky black read as black. This is the commonest mistake with this gene.
Palomino read as flaxen chestnut, and the other way round
Cremello or perlino read as white or albino. These horses have cream hair and still make a little pigment. True white horses owe their colour to other genes.
Buckskin read as bay dun, or the two present together
Grey, which slowly strips any of these colours away

Health. No disease is linked to Cream. Blue eyes and pink skin are part of the colour, not a defect, and need no treatment.

Trasmissione

Cream shows with one copy, and shows more with two. The gene is not on a sex chromosome, so colts and fillies are the same.

What each mating can produce:

N/N x N/N gives 100% N/N
N/N x N/Cr gives 50% N/N, 50% N/Cr
N/N x Cr/Cr gives 100% N/Cr
N/Cr x N/Cr gives 25% N/N, 50% N/Cr, 25% Cr/Cr
N/Cr x Cr/Cr gives 50% N/Cr, 50% Cr/Cr
Cr/Cr x Cr/Cr gives 100% Cr/Cr

Prevenzione

The result lets you plan which dilute colours a mating can give.

To guarantee a dilute foal. Use a parent with two copies.

To get a palomino. The foal needs one Cream copy on a chestnut base. Cremello put to chestnut gives only palominos.

To get a buckskin. The foal needs one Cream copy on a bay base. Cremello put to bay is the most efficient route, and the outcome then depends on the bay parent's own base colour genes.

To avoid a double dilute. Mate a Cream carrier only with a partner that tested negative.

Risultati

Il test del DNA verifica la presenza del gene di diluizione Cream e presenta i risultati come uno dei seguenti:

N/ - Non diluito. I colori di base sono nero, baio o castano, in assenza di altri geni modificatori.
N/Cr – Diluito. Eterozigote, una copia dell'allele Cream (Cr). Castano è diluito in palomino; baio è diluito in buckskin e nero è diluito in smoky black. Questi colori possono essere ulteriormente modificati dall'azione di altri geni.
Cr/ - Doppia diluizione, due copie dell'allele Cream (Cr). Il castano è diluito in cremello; il baio è diluito in perlino e il nero è diluito in smoky cream.

Requisiti di esempio

30 a 40 - radici dei capelli - busta o 5 mL - sangue - tubo K3 EDTA

Tempi di consegna

Da 2 a 5 giorni lavorativi

Come funziona

🛒 Acquista il test: seleziona e acquista il test online.

📧 Ricevi le istruzioni: dopo la conferma del pagamento, ricevi via e-mail le istruzioni per il prelievo del campione.

📄 Compila il modulo: stampa e compila il modulo di invio campioni con l’identificazione dell’animale.

✨ Raccogli il campione: strappa da 20 a 40 radici di pelo con il bulbo e incollale nell’area indicata del modulo, oppure chiedi al tuo veterinario di prelevare il sangue in una provetta K3-EDTA.

📮 Invialo al laboratorio: Equigerminal, S.A., HIESE, Rua da Quinta do Sobreiro, 25, Quinta Vale do Espinhal, 3230-343 Penela, PORTUGAL.

📊 Ricevi il referto: il referto certificato viene emesso non appena l’analisi è convalidata.

Domande frequenti

What does this test not detect?
It reads Cream only. It does not read Pearl, Sunshine or Snowdrop, three other dilutions in the same gene. It does not test Champagne, Silver, Dun, Grey, Mushroom, any white pattern or the base colour. It says nothing about health, performance or temperament.

At what age can a horse be tested?
Any age. The genotype is fixed at conception and never changes. A foal coat is a poor guide to dilution, so testing early makes sense.

Should I also test for Pearl?
Yes, if the horse is pale and the Cream result does not explain it, or if it is of Iberian or stock-horse origin, where Pearl occurs and can imitate Cream. Where relevant, test Champagne too.

For Professionals

Genetic and clinical detail for veterinarians, geneticists and laboratories.

Gene / locus: SLC45A2 (solute carrier family 45 member 2; formerly MATP), Cream (C) locus, ECA21.
Variant: C^Cr allele, missense. HGVS (EquCab3.0): NC_009164.3:g.31690653G>A; XM_001498110.4:c.601G>A; XP_001498160.2:p.(Asp201Asn).
Alternative designations: published as exon 2 c.457G>A, p.Asp153Asn (Mariat et al., 2003). The genomic position is the same; c. and p. numbering differ because OMIA uses a more recent RefSeq transcript.
Variant identifiers: rs1140980396 (EVA); OMIA variant 153.
Variant classification: not currently evaluated (ISAG/AVCG, per OMIA).
Mechanism: SLC45A2 encodes a transporter the melanocyte needs to function; the Cream variant reduces the pigment deposited in the hair.
Inheritance: autosomal incomplete dominant (dosage effect): one copy dilutes phaeomelanin, two copies dilute phaeomelanin and eumelanin. OMIA code: A (autosomal).
Breeds with documented carriers or cases: OMIA variant record lists Quarter Horse, Morgan and Welsh Pony. Avila et al. (2022) found Cream in 25 of 28 breeds: Andalusian, Appaloosa, Arabian, Connemara Pony, Dutch Warmblood, Gypsy Cob, Gypsy Vanner, Icelandic Horse, Lusitano, Miniature Horse, Missouri Fox Trotter, Morgan Horse, Mustang, Norwegian Fjord Horse, Oldenburg, Paint Horse, Pony of the Americas, Pura Raza Espanola, Quarter Horse, Rocky Mountain Horse, Shetland Pony, Standardbred, Tennessee Walking Horse, Thoroughbred and Welsh Pony. Not detected in Hanoverian (n=31), Knabstrupper (n=71) or Percheron (n=37); absence in a sample does not prove absence in the breed.
Allele / carrier frequencies: allele frequency from 42% in the Lusitano (n=102) to 1.3% in the Standardbred (n=39), Iberian breeds among the highest (Avila et al., 2022; 11,281 horses tested, 6,878 after removing close relatives). The authors note ascertainment bias, as samples were submitted for colour testing, so figures for less-sampled breeds may be overestimated.
Clinical / diagnostic notes for veterinarians: SLC45A2 carries at least four equine dilution alleles: Cream, Pearl (Sevane et al., 2019), Sunshine (Holl et al., 2019) and Snowdrop (Bisbee et al., 2020).

Key references:

Mariat D, Taourit S, Guerin G (2003). A mutation in the MATP gene causes the cream coat colour in the horse. Genet Sel Evol 35:119-133. doi:10.1051/gse:2002039. PMID 12605854.
Locke MM, Ruth LS, Millon LV, Penedo MCT, Murray JD, Bowling AT (2001). The cream dilution gene, responsible for the palomino and buckskin coat colours, maps to horse chromosome 21. Anim Genet 32:340-343.
Holl HM, Pflug KM, Yates KM, Hoefs-Martin K, Shepard C, Cook DG, Lafayette C, Brooks SA (2019). A candidate gene approach identifies variants in SLC45A2 that explain dilute phenotypes, pearl and sunshine, in compound heterozygote horses. Anim Genet 50:271-274. doi:10.1111/age.12790. PMID 31006892.
Avila F, Hughes SS, Magdesian KG, Penedo MCT, Bellone RR (2022). Breed distribution and allele frequencies of base coat color, dilution, and white patterning variants across 28 horse breeds. Genes 13:1641.